Figure 3.

PDH1 is a retrograde regulated gene and may function in propionate metabolism. (A) Northern blots showing increased abundance of PDH1 and CIT2 transcripts in ρ^o compared with ρ⁺ cells at three different cell densities. (B) Growth of wild-type (WT) and pdh1Δ cells on YNB 1% casein medium with or without propionate and containing limiting amounts of raffinose as indicated. (C) Growth of wild-type (WT) and pdh1Δ cells on YNB, 1% casein, 0.05% raffinose medium with or without propionate or acetate as indicated.