Figure 5. Skin infection with LAC induces IFN-γ in a saeR/S-dependent manner.
Mice were inoculated subcutaneously with LAC, LACΔsaeR/S (1×107 cfu) or DPBS for 8 hours. Infected tissues were excised and homogenized for bacterial load enumeration and IFN-γ concentration measurements. (A) Bacterial burden at the site of infection. (B) IFN-γ concentration (pg/g tissue) in affected tissues. *P<0.05 compared to MW2 in each panel as determined by t test. Results are from 3 biological replicates of 3 mice/group (n = 9) and 2 mice/PBS group (n = 6). NS = not significant; N/D = not detectable.