Skip to main content
. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Prostate Cancer Prostatic Dis. 2011 Jan 11;14(2):97–104. doi: 10.1038/pcan.2010.52

Figure 3. Micrographs of RM-9 cells 24 hours following calcitriol, cryo, or combination treatment.

Figure 3

Phase contrast images (10X) were acquired 24 hours post-freeze treatment. Samples were treated with 50, 75, 100, or 150nM calcitriol and exposed to a single, 10 minute, −15°C freeze. Percentages indicate correlating viability data (Fig 2). Calcitriol pretreatment [75nM-100nM] in conjunction with −15°C cryoexposure produces a loss of cells, reduced attachment and morphology alterations.