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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Aging Cell. 2011 Mar 31;10(3):429–437. doi: 10.1111/j.1474-9726.2011.00679.x

Figure 4. Aortic oxidative stress, superoxide production and oxidant/antioxidant enzymes.

Figure 4

(A) Nitrotyrosine in aorta of young and old control (YC and OC) and young and old nitrite supplemented (YN and ON) mice. Data are expressed relative to GAPDH and normalized to YC mean value. Representative western blot images below. (B) Mean electron paramagnetic resonance (EPR) signal for superoxide from aortic rings. (C) Aortic superoxide dismutase (SOD) enzymatic activity. (D) Protein expression of p67 subunit of NADPH oxidase in aorta of young and old control (YC and OC) and young and old nitrite supplemented (YN and ON) mice. Data are expressed relative to GAPDH and normalized to YC mean value. Representative western blot images below. Values are mean ± SEM. (n = 6 – 9 per group) * p < 0.05 vs. YC.