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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Structure. 2011 May 11;19(5):700–710. doi: 10.1016/j.str.2011.02.011

Figure 4. ITC profiles of Zn2+ binding to the soluble and full-length StZntB.

Figure 4

The binding isotherms were obtained as described in Methods with correction for heat of dilution at 25°C. Zn2+ binding to the soluble domain and full-length StZntB was derived from the non-linear least square fit of the isotherms. The isotherm of the soluble domain could be best fitted to the two site sequential binding model while the full-length StZntB was best fitted to a three site binding model. See also Supplementary Figure 4.