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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Feb 3;50(10):2294–2296. doi: 10.1002/anie.201006460

Figure 3.

Figure 3

Translocation of LFN variants through PA pore is hindered by cysteic acid. a) Chemical framework of SSv5 and SSv6 (X = cysteic acid). b) The fraction ion conductance block of PA pore by SSv5 and SSv6 at Δ Ψ = 20 mV. c) Translocation of LFN variants through PA pore in response to a pH gradient of ~2 units (cis pH 5.5; trans pH 7.5) at Δ Ψ = 20 mV. d) Acid triggered translocation of LFN-DTA variants through PA pore in response to a pH gradient of ~2 units (cis pH 5.5; trans pH 7.5) at Δ Ψ = 20 mV. e) Fraction protein synthesis inhibition by LFN-DTA variants in CHO-K1 cells. The procedure used was identical to that described in Figure 2e.