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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Immunol Cell Biol. 2006 Apr;84(2):203–208. doi: 10.1111/j.1440-1711.2006.01418.x

Figure 2.

Figure 2

Glucose-regulated protein 170 (GRP170) upregulates expression of (a) MHC class II, (b) CD86 and (c) CD40 molecules in dendritic cells (DC). Bone marrow-derived DC (106/mL), derived from C57BL/6 mice, were cultured with mouse recombinant GRP170 (200 μg/mL), LPS (0.1 μg/mL), or luciferase at the same molar ratio as GRP170 (70 μg/mL) in RPMI-1640 supplemented with 10% FBS for 22 h followed by blocking of the Fc receptors using rat antimouse CD16/CD32 antibody. Cells were stained with FITC-conjugated rat antimouse antibodies and subjected to flow cytometry analysis. Isotype control antibody (1 μg/200 μL) was used as control and revealed <12 mean fluorescence intensity (MFI). Data are presented as MFI of triplicate experiments.