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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Immunol Cell Biol. 2006 Apr;84(2):203–208. doi: 10.1111/j.1440-1711.2006.01418.x

Figure 3.

Figure 3

Glucose-regulated protein 170 (GRP170) augments expression of CD40 molecules in the B16F10 melanoma cell line. B16F10 (106/mL) was cultured in the presence or absence of recombinant GRP170 (200 μg/mL) or LPS (0.1 μg/mL) in RPMI-1640 (37°C, 5% CO2) for 22 h. Tumour cells were stained with FITC-conjugated rat antimouse CD40 antibody. Cells were then subjected to flow cytometry analysis after fixing with 1% formaldehyde. Isotype control antibody (1 μg/200 μL) was used as control and revealed <12 mean fluorescence intensity (MFI). Data are presented as MFI of triplicate experiments.