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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Immunol Cell Biol. 2006 Apr;84(2):203–208. doi: 10.1111/j.1440-1711.2006.01418.x

Figure 4.

Figure 4

Glucose-regulated protein 170 (GRP170) induces dendritic cells (DC) to secrete pro-inflammatory cytokines. Mouse bone marrow-derived DC (106/mL) were cultured in the presence or absence of recombinant GRP170 (200 μg/mL), LPS (0.1 μg/mL), or a control protein luciferase (70 μg/mL) for 22 h. Supernatants were collected and subjected to multiplex cytokine assay using the cytokine-specific antibody-coated beads (anti-IL-6, anti-IL-12 and anti-TNF-α), biotinylated detection antibodies (anti-IL6, anti-IL12 and anti-TNF-α), and phycoerythrin (PE)-avidin. Data are presented as the mean of triplicate experiments. (a) IL-6, (b) IL-12, (c) TNF-α.