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(Lignans). High performance liquid chromatography (HPLC) of the bioactive fraction was performed using methanol:water gradient over a period of 70 min: column; RP-18 (E. Merck, 5 μm, 4.0 × 250 mm); Flow rate 0.6 ml min−1; UV 290 nm.
| Time (min) | Percentage of A (water) | Percentage of B (methanol) |
|---|---|---|
| 00.01 | 65.00 | 35.00 |
| 60.00 | 35.00 | 65.00 |
| 70.00 | 65.00 | 35.00 |
