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(Flavonoids). HPLC of the bioactive fraction was performed using acetonitrile containing 0.05% of tri-flouro acetic acid (TFA):water containing 0.05% of TFA gradient over a period of 70 min: column; RP-18 (E. Merck, 5 μm, 4.0 × 250 mm); Flow rate 1.0 ml min−1; UV 250 nm.
| Time (min) | Percentage of A (water) | Percentage of B (acetonitrile) |
|---|---|---|
| 00.01 | 88.00 | 12.00 |
| 25.00 | 79.00 | 21.00 |
| 30.00 | 75.00 | 25.00 |
| 35.00 | 25.00 | 75.00 |
| 60.00 | 00.00 | 100.00 |
| 70.00 | 88.00 | 12.00 |
