Skip to main content
. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Mar 17;370(1):38–43. doi: 10.1016/j.bbrc.2008.03.026

Fig. 4.

Fig. 4

Gene knock-down of DFNA5 in MDA-MB-231 and MCF-10A. (A) Gene knock-down of DFNA5 increased the number of invading cells. Cell growth for 16 h determined by MTT assay was not significant (data not shown). Cont, control. Colony focus assay was performed after transfection of DFNA5 siRNAs into MDA-MB-231 (B) or MCF-10A (C). No colonies from control siRNA-transfected MCF-10A cells were observed. Values are expressed as means ± SD and are derived from two independent experiments done in triplicate. *P < 0.05. (D) Cell growth in MDA-MB-231 after transfection of DFNA5 siRNA-#2. MTT assay results are expressed as absorbance (570 nm), and experiments were performed twice in triplicate. (E) Since no commercial anti-DFNA5 antibody is currently available, gene knock-down was confirmed by RT-PCR in each cell line. Left, MDA-MB-231, Right, MCF-10A. β-actin was used as a loading control.