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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Curr Top Med Chem. 2011;11(6):629–647. doi: 10.2174/1568026611109060629

Figure 2.

Figure 2

Identification of RvE1 receptor.

(A) Functional screening for RvE1 receptors. HEK293 cells cotransfected with pNF-κB-luciferase and pcDNA3-GPCRs were exposed to RvE1 (10 nM) and TNF-α (inset) Phylogenetic tree representing amino acid sequence similarities between the human LXA4 receptor (ALX), leukotriene B4 receptor (BLT1) and related GPCRs.

(B) Receptor dependence. RvE1 inhibits luciferase activity in a concentration-dependent manner on cells transfected with ChemR23 but not mock transfected cells.

(C) Ligand specificity. Cells transfected with pcDNA3-ChemR23 were exposed to 100 nM of each compound. RvE1 showed ~60%, while EPA and 18-HEPE do not have inhibition of NF-κB luciferase activity,