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BALF-induced fibroblast chemotaxis is similar in WT, β-arrestin1−/−, and β-arrestin2−/− mice. Primary lung fibroblasts were plated in serum-free DMEM onto modified Boyden chambers with a fibronectin-coated filter. BALF from bleomycin-treated mice was diluted in serum-free DMEM and added to the bottom chamber. After 4 hours, the number of fibroblasts that migrated to the other side of the filter was counted. Data represent mean ± SE of four independent experiments.
