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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Sci Transl Med. 2011 Mar 16;3(74):74ra23. doi: 10.1126/scitranslmed.3001564

Figure 6.

Figure 6

β-arrestin1 and β-arrestin2 are required for primary lung fibroblast invasion. (A) Primary lung fibroblasts were isolated from untreated mice and mice 7 days after bleomycin treatment. After 3 passages, the fibroblasts were loaded onto a matrigel-coated filter, and after 24 hours, the number of fibroblasts that invaded through the matrix was counted. Data represent mean ± SE of four independent experiments. *P < 0.05 compared to all other groups by the Tukey-Kramer test for multiple comparisons. (B) Primary lung fibroblasts isolated from bleomycin-treated mice were transduced with siRNA specific to β-arrestin1, β-arrestin2, or nonspecific control (Ctl). Invasive capacity of the fibroblasts was assessed with the Matrigel invasion assay. Quantitative analysis of knockdown experiments demonstrated decreased invasion after knockdown of both β-arrestin1 and β-arrestin2. **P < 0.01. The experiments were repeated three times.