Figure 3. Klf2 and Klf4 response of WT and Tg737^orpk/orpk EC to shear stress.

(A,B) Relative mRNA expression of Klf2 and Klf4 in WT and Tg737^orpk/orpk cells under 0.5 Pa shear stress with and without pan-Tgfβ neutralizing antibody (α-Tgfβ) and SB431542 (SB). Expression is normalized to GAPDH and relative to static shams, as represented by the dashed line. (C) Western Blot analysis and quantification of Klf4 protein levels in Tg737^orpk/orpk cells under static conditions, and under 0.5 Pa shear stress with and without SB.