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. 2011 Mar 11;25(5):856–867. doi: 10.1038/leu.2011.28

Figure 2.

Figure 2

Nutlin-3a induces cell cycle arrest and/or cell death in diffuse large B-cell lymphoma (DLBCL) associated with t(14;18)(q32;q21) through activation of the p53 pathway. (a) At 24 h after treatment with 2 μ nutlin-3a in DLBCL cell lines with wild-type (wt) p53 resulted in G1 and G2/M phase cell cycle arrest with decreased S phase (DoHH2), cell cycle arrest in G1 phase, decreased S phase accompanied by cell death (MCA and OCI-Ly3) or cell death without cell cycle arrest and decreased S phase (OCI-LY10 and EJ). No cell death or cell cycle arrest was seen in cells that lacked p53 or with mutant p53 (Pfeiffer, MS and BJAB). (b) Western blot analysis at 24 h after treatment with 5 μ nutlin-3a showed increased expression of p21, in parallel with increased p53 levels in cells with wt p53 along with upregulation of MDM2 and downregulation of MDMX. No such changes were detected in Pfeiffer, BJAB and MS cells treated with nutlin-3a. (c) At 12 h following treatment with nutlin-3a, DoHH2 and MCA cells had increased levels of p53 visualized by immunofluorescence. p53 localization was predominantly nuclear. The 4',6-diamidino-2-phenylindole was used as counterstain of the nuclei.