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The effect of P. minima chloroform extract (2.80 μg/mL at 72 h) on NCI-H23 cells for 24 h and subjected to Annex-V-FLUOS kit (Roche, Germany). A weakly scattered annex in V staining pattern of cell surface was evident in most of the cells (thin arrows). Only a few of homogenous-high intensity of annex in V fluorescence cells (thick arrow) and both annex in V-propidium iodide stained cells (green and red fluorescence) (dotted arrows) were also observed.
