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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Pediatr Res. 2011 Jun;69(6):479–484. doi: 10.1203/PDR.0b013e318217a0df

Figure 1.

Figure 1

α1D Ca2+ channel mRNA and proteins levels during heart development using real-time PCR and Western blot respectively. A: α1D Ca2+ channel mRNA during rat heart development by real-time RT-PCR using 18sRNA as internal control. Value of the fetal stage was set at 100 for easy comparison. * indicates p<0.05 compared to adult stage. F.A: fetal atria; N.A: neonatal atria; A.A: adult atria. B: Representative Western Blot experiment using anti-α1D Ca2+ channel antibody. Total membrane protein (100 µg) was loaded in each lane. Lane 1, fetal stage; Lane 2, neonatal stage; lane 3, 6-weeks atria; lane 4, 6-week ventricles; lane 5, 6-months atria; lane 6, 6-months ventricle and lane 7, negative control (Anti-α1D Ca2+ channel antibody preincubated with antigenic peptide using membrane protein from fetal stage). Panel B, lower part, shows non-specific protein bands observed in each lane, indicating that absence of α1D protein in the adult stage is not secondary to loading error.