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Synaptotagmin I C2A (A) and C2AB (B) fluorescence intensity ratios of ordered lipid domain regions over disordered lipid non-domain regions after binding to asymmetric two-phase bilayers in the presence of 1 mM Ca2+ and increasing concentrations of bPIP2 in the inner leaflet monolayers as indicated on the x-axis. All outer leaflet monolayers are composed of bPC/bSM (1:1) plus 20% cholesterol and supported on a polymer cushion. Error bars indicate standard errors.
