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Activation of transcription factor Mga2p is inhibited in doa4Δ cells. N-terminally Myc-tagged Mga2p was expressed in wt and doa4Δ cells alone in panel A or along with Ub and BMV 1a in panel B. Accumulation of Mga2p p120 and p90 was detected with an anti-Myc monoclonal antibody by Western blotting.
