Table 1.
Fusion (F) protein cleavage sites of different APMV serotypes that were incorporated into the F protein cleavage site of APMV-2
| Recombinant virus | Fusion protein cleavage sitea |
|---|---|
| rAPMV-2 | KPASR↓F |
| rAPMV-2 (F-L) | KPASR↓L |
| rAPMV-2 (type 1v) | RRQKR↓F |
| rAPMV-2 (type 1av) | GRQGR↓L |
| rAPMV-2 (type 1 Africa) | RRRRR↓F |
| rAPMV-2 (type 3) | RPRGR↓L |
| rAPMV-2 (type 4) | DIQPR↓F |
| rAPMV-2 (type 5) | KRKKR↓F |
| rAPMV-2 (type 6) | APEPR↓L |
| rAPMV-2 (type 7) | LPSSR↓F |
| rAPMV-2 (type 8) | YPQTR↓L |
| rAPMV-2 (type 9) | IREGR↓I |
a
Segment III (as shown in Fig. 1) in pAPMV-2 was mutated by overlap PCR to change the F protein cleavage site, digested using NotI and PacI sites, and subcloned into the full-length cDNA to generate rAPMV-2 F protein cleavage site mutants. The basic amino acids (K and R) at the F protein cleavage site are shown in bold. The arrow indicates the site of cleavage.