Fig. 3.
BCoV “long” (i.e., 16-nt-extended) SLIV immediately supports MHV wt-like virus replication in the MHV background, but the BCoV 32-nt inter-stem-loop domain requires blind passage adaptation before progeny appear. (A) Scheme showing chimera constructs. Note that the long form of SLIV is depicted. (B) Plaque phenotypes of wt MHV and the identified chimeras. (C) Growth kinetics of wt MHV and B174–226/M chimera (left panel) and Northern analysis showing RNA patterns (right panel). (D) Growth kinetics of wt MHV and chimera B1–141, 174–226/M (left panel) and Northern analysis showing RNA patterns (right panel). For growth kinetics, the standard error of three measurements is shown. For Northern analyses, an MHV 3′-UTR-specific probe was used.