Fig. 2.

(A) Virucidal activity of TSC. BVDV was incubated 10 min at rt in contact with 10× and 20× EC₅₀ (17 μM and 34 μM, respectively) of TSC. The residual infectious viruses were titrated by plaque assay in MDBK cells. (B) Effect on viral replication of the treatment of MDBK cells before infection. Subconfluent MDBK cells monolayers were treated with 10× and 20× EC₅₀ of compound and further incubated for 7 h at 37°C before infection with BVDV. After removal of the medium, treated and untreated cells were infected with 100 PFU of BVDV. Following 72 h of incubation at 37°C, cells were fixed and stained, and the plaques counted.