Fig. 3.

Effect of TSC during viral adsorption-penetration. MDBK cells were infected with BVDV (MOI of 3). After 2 h at 37°C, the inoculum was removed, and cultures were further incubated for 48 h, when cell viability was determined by the MTS/PMS method. Three different treatments with the compound (34 and 17 μM) were carried out: (i) only during (white bars), (ii) only after (gray bars), and (iii) during and after the 2-h incubation period (black bars). Mock-infected and infected cells without compound were added as controls (open bars).