Fig. 6.

Effect of TSC addition on viral RNA synthesis. (A) Kinetics of BVDV RNA synthesis. MDBK cells were infected with BVDV (MOI of 3), and the ivRNA was measured by real-time PCR at different times p.i. (the inset presents the ivRNA levels during the period from 4 to 12 h, on a more detailed scale). (B) Simultaneously, MDBK cells were infected with BVDV (MOI of 3), and 34 μM TSC was added every 2 h during the first 12 h p.i. The incubation period continued until 24 h p.i., when intracellular RNA was extracted and ivRNA measured by real-time RT-PCR (the inset presents the 24-h ivRNA levels during which the compound was added from 0 to 12 h, on a more detailed scale). An infected untreated control (IUT) was included, and its 24-h-p.i. ivRNA was measured. Values are expressed as 1/ΔCT, where ΔCT = CT BVDV 5′ NTR − CT β-actin. NIUT, nontreated uninfected control.