Fig. 7.
Effect of TSC on BVDV RC activity. MDBK cells were mock infected or infected with BVDV. After 24 h, RCs were collected, and RNA synthesis was evaluated by the in vitro incorporation of [α-32P]GTP into vRNA. Reaction product of the RC assay was denatured and analyzed on 3% PAGE-7 M urea. (A) RNA product from infected and mock-infected MDBK cells. (B) RNA product from RCs of wt BVDV-infected cells in the presence of a series of TSC concentrations. (C) RNA product from RCs of wt BVDV- or BVDV-TSCr T2-infected cells in the presence or absence of TSC (80 μM). Similar results were obtained for the RCs BVDV-TSCr T1-infected cells.