Table 1.
Sequences and positions in the genome of BVDV NADL of oligonucleotides used for real-time RT PCR and PCR amplification of the nonstructural protein coding region
| Oligonucleotide name | Sequence 5′–3′ | Position in the genomea |
|---|---|---|
| 5′NTR Fw | GAGGCTAGCCATGCCCTTAGT | 98–118 |
| 5′NTR Rv | TCGAACCACTGACGACTACCCT | 185–206 |
| NS2-3 Fw 0 | AATACTGGTTTGACCTGGAG | 3435–3454 |
| NS2-3 Rv 0 | CGCCAGTTCTTTCAGTTCAGT | 7646–7666 |
| NS2-3 Fw 1 | CGCTGAGTCCATATTAGTGGT | 3481–3501 |
| NS2-3 Rv 1 | ACTGAACTTATCCCGCCT | 5605–5622 |
| NS2-3 Fw 2 | CTGCCGTGTGTAAGAAGAT | 5427–5445 |
| NS2-3 Rv2 | GTCTTCTAGTCTCTGGTCCT | 7575–7594 |
| NS4A-B Fw9 | TAGACTGGCCTGATCCTG | 7401–7418 |
| NS4A-B Rv9 | GTTGGCAATCTGATCCTCT | 8826–8844 |
| NS5A Fw1 | CTGTATGGGGTTTACTACAAAGG | 8567–8589 |
| NS5A Rv3 | TTGGTAAGCTGATGCCATGTG | 10295–10315 |
| NS5B Fw11 | AGGAGATGTTGGAGAGGTAA | 10090–10109 |
| NS5B Rv16 | TCTTCTTGAGGTGGACGG | 12417–12434 |
a
According to BVDV NADL complete genome. GenBank accession no. AJI33738.1.