Table 1.

Primers used for RT-PCR isolation and nucleotide sequence analysis of the RRV E2 gene from a small-plaque variant of RRV-T48

Primer	Polarity	Position (5′)a	Oligonucleotide sequence (5′–3′)b	Product size (bp)
P1	+	8545	GAAACAGATCACGCCACCGC	1,289
P2	−	9834	CTGCGTTCGCCCTCGGTGCG
P3	+	8946	GTCCAATACAAGCACGACCC
P4	−	9454	TAGGAGAAGAGCGTCGGATG
P5	−	10035	GAATTGTGGCTGTGTGCTCGb	1,490 (with P1)

^aPosition of primer in complete RRV genome (refer to reference 10).

^bPrimers used for RT-PCR amplification of RRV E2. The E2 gene is 1,268 bp in length (position 8566 to 9834) and is situated between the E3 and 6K coding regions of the RRV genome (10).