Table 2.
Primers used for nucleotide sequence analysis of the RRV nsP1 to nsP4 genes from RRVPERS
| Primer | Polarity | Position (5′) | Oligonucleotide sequence (5′–3′) | Product size (bp) |
|---|---|---|---|---|
| P1 | + | 7 | GGACGTGTGACATCACCGTTC | 655 |
| P2 | − | 662 | GGAATACGTTGGGTATGCGCC | |
| P3 | + | 603 | GGATTCGACACCACCCCATTC | 650 |
| P4 | − | 1253 | CTTGCTGAAAGCTTGGGCCAC | |
| P5 | + | 1147 | TACTGGTGGGGTTGAACCAAC | 697 |
| P6 | − | 1844 | TCCTGAGTGCGTCATGATTGTC | |
| P7 | + | 1744 | CGCGTACCTGATCTTGTCTC | 708 |
| P8 | − | 2452 | TGACAGGCAAATGCTTCGTCC | |
| P9 | + | 2352 | AGAGGACTGGATGTAACAGCC | 630 |
| P10 | − | 2982 | ACACCTTTATCCACGGGTCTC | |
| P11 | + | 2939 | GCTGGTGTGGAAAACACTGTC | 641 |
| P12 | − | 3580 | TGGTATCCGGGAAGCTTCTTC | |
| P13 | + | 3361 | GCGAAGTCGCCCGTAAGTTTG | 776 |
| P14 | − | 4137 | CATTGACAACCGCCTCTTCGG | |
| P15 | + | 4076 | ACCCTCATACCGTGTGCGTAG | 756 |
| P16 | − | 4832 | CGAATCGGCGTCCTCAACAG | |
| P17 | + | 4795 | GCATCAGAACGAAATGCCCTG | 777 |
| P18 | − | 5572 | TCAACCGGCTCGCGCATTTTG | |
| P19 | + | 5514 | CTGAAGATCTGGAGGTACTCAC | 827 |
| P20 | − | 6341 | TAGAGTGGGTAGCTCTCTCATC | |
| P21 | + | 6242 | CGCGGTCCCATCACCATTTC | 677 |
| P22 | − | 6919 | ATTTCCCCGAACGCTGCCTC | |
| P23 | + | 6840 | TTGACGGCACTGATGCTGTTG | 680 |
| P24 | − | 7520 | TTTAGGACCGCCGTAGAGGTG | |
| P25 | + | 7311 | CAAGATGAAGATCGTAGGCGTG | 439 |
| P26 | − | 7750 | CTGTTTGGTGGTTAGTGCAGAG |