Table 3.
Mean RRV titers in RAW 264.7 murine macrophages over 21 days p.i.a
| Day p.i. | Mean RRV titer (log10 PFU/ml) ± SEM |
|---|---|
| 1 | 4.77 ± 0.02 (0 small plaques) |
| 2 | 5.27 ± 0.16 (0 small plaques) |
| 5 | 5.34 ± 0.11 (27, 5.8, 8.5b) |
| 14 | 5.93 ± 0.14 (89, 93, 91b) |
| 21 | 4.01 (80b,c) |
a
Cells were infected at an MOI of 0.1 on day 0 (n = 3) and cultured with 5 ng/ml LPS. Virus used in this experiment was derived from the RRV-T48 genetic clone (RR64).
b
Proportion(s) (%) of small RRV plaques observed on Vero cell monolayers after RAW 264.7 macrophage infection (n = 3 except where otherwise noted) over a 21-day period. Parent virus (RRV-T48) presents as a “large” plaque on Vero cell monolayers (Fig. 1). At days 1 and 2 post-RRV-T48 infection, only large plaques were observed.
c
n = 1.