Fig. 7.
The effect of CAV-RTA capsid mutations on viral RT competence. (A) Virus recovered from lungs of mice inoculated with 2 × 105 PFU of CAV-RTB up to 10 h (middle) and 96 h (right) after i.t. infection. Groups of male hICAM-1 tg mice or non-tg littermates (n = 4) were inoculated i.t. with CAV-RTB as indicated and tested for virus recovery from lungs at the intervals shown. The values represent the average recovered amounts of virus from the animals in each group. The bars indicate the standard deviations. (B) (Left) Erk1/2 and Akt(Thr308) phosphorylation after binding of CAV-RTA or -RTB to serum-starved HeLa H1 cells (the data are a representative series of results from three independent assays). (Right) Densitometry of p-Erk1/2 immunoblots. The mean values from results of two independent assays are shown; bars indicate standard deviations.