Fig. 4.
CWD PrPSc replication is selectively inhibited by sorption to soil. (A) Western blot analysis of soil-bound CWD agent-infected samples prior to (−) and after (+) two rounds of PMCA. (B) Quantification (n = 3) of PrPSc abundance following one or two rounds of PMCA of samples seeded with either uninfected brain, CWD agent-infected elk (CWD-elk) brain homogenate, or CWD-elk brain homogenate bound to soil. Amplified PrPSc abundance was normalized to the second-round unbound (BH) sample. Bent, bentonite.