Fig. 2.

Cav-1 modulates gene expression under the control of the HIV-1 LTR in the presence and absence of Tat. (A) GFP under the control of CMV promoter (pCMV-GFP) or the HIV LTR (pLTR-GFP) was cotransfected with pCZ-Cav-1 or pCZ-vector in the presence or absence of Tat (pTatZ) into HEK 293T cells. The cells were washed with PBS twice and subjected to flow cytometry to detect GFP fluorescence intensity. Results are shown as means ± SD of three determinations. (B) The cells were also harvested and subjected to Western blot analysis to determine the expression levels of Cav-1, Tat, and β-actin.