Fig. 4.

Detection and quantification of viral RNA loads in livers of pigs inoculated with the wild-type pSHEV-3 and HEV mutants. HEV GE titers were determined from samples harvested at necropsies on days 21 (A), 28 (B), and 56 (C). Data are shown as log₁₀ of GE per mg of liver. The dotted line represents the limit of the sensitivity of the qRT-PCR assay (1.53 log₁₀ GE/mg).