Fig. 5.

Detection and quantification of viral RNA loads in bile samples from pigs inoculated with the wild-type pSHEV-3 and HEV mutants. HEV GE titers were determined from samples harvested at necropsies on days 21 (A), 28 (B), and 56 (C). Data are shown as log₁₀ of GE per ml of bile. The dotted line represents the limit of the sensitivity of the qRT-PCR assay (2.90 log₁₀ GE/ml).