Table 1.
HEV RNA in feces and sera of specific-pathogen-free pigs intrahepatically inoculated with capped RNA transcripts from wild-type pSHEV-3 and mutant rF51L, rT59A, rS390L, and pSHEV-1 infectious clones
| Group | Inoculum | Sample | No. of positive pigs/no. tested at dpi: |
Total no. positive/no. tested | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 7 | 14 | 21 | 28 | 35 | 42 | 49 | 56 | ||||
| A | pSHEV-3 | Feces | 0/10 | 3/10 | 8/10 | 10/10 | 7/7 | 4/4 | 4/4 | 4/4 | 4/4 | 10/10 |
| Serum | 0/10 | 0/10 | 1/10 | 2/10 | 6/7 | 2/4 | 2/4 | 2/4 | 1/4 | 7/10 | ||
| B | rF51L | Feces | 0/10 | 2/10 | 2/10 | 5/10 | 5/7 | 4/4 | 3/4 | 3/4 | 4/4 | 7/10 |
| Serum | 0/10 | 0/10 | 0/10 | 0/10 | 2/7 | 1/4 | 1/4 | 1/4 | 1/4 | 2/10 | ||
| C | rT59A | Feces | 0/10 | 0/9 | 0/9a | 3/9b | 5/6 | 3/3 | 0/3 | 0/3 | 0/3 | 8/10 |
| Serum | 0/10 | 0/9 | 0/9 | 0/9 | 0/6d | 0/3 | 0/3 | 0/3 | 1/3 | 1/10 | ||
| D | rS390L | Feces | 0/10 | 0/10 | 0/10a | 3/10b | 7/7 | 4/4 | 4/4 | 0/4 | 0/4 | 8/10 |
| Serum | 0/10 | 0/10 | 0/10 | 1/10 | 1/7 | 3/4 | 0/4 | 0/4 | 0/4 | 5/10 | ||
| E | pSHEV-1 | Feces | 0/10 | 0/10 | 0/10a | 1/10b | 1/7 | 0/4 | 0/4 | 0/4 | 0/4 | 1/10 |
| Serum | 0/10 | 0/10 | 0/10 | 0/10 | 0/7d | 0/4 | 0/4 | 2/4 | 1/4 | 2/10 | ||
| F | PBS | Feces | 0/10 | 0/10 | 0/10a | 0/10b | 0/7c | 0/4 | 0/4 | 0/4 | 0/4 | 0/10 |
| Serum | 0/10 | 0/10 | 0/10 | 0/10 | 0/7 | 0/4 | 0/4 | 0/4 | 0/4 | 0/10 | ||
Group with a statistically significant difference in the incidence of fecal virus shedding compared to the wild-type group at 14 dpi (P < 0.05).
Group with a statistically significant difference in the incidence of fecal virus shedding compared to the wild-type group at 21 dpi (P < 0.05).
Group with a statistically significant difference in the incidence of fecal virus shedding compared to the wild-type group at 28 dpi (P < 0.05).
Group with a statistically significant difference in the incidence of viremia compared to the wild-type group at 21 dpi (P < 0.05).