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Valproic acid activates the expression of KSHV ORF50 but not that of the EBV homolog BRLF1. HH-B2 cells (harboring latent KSHV) and HH514-16 cells (harboring latent EBV) were either left untreated or treated with NaB, TSA, or VPA. Cells were harvested 8 h after treatment and total RNA extracted. Relative levels of ORF50 and BRLF1 mRNAs were measured by qRT-PCR using the standard-curve method.
