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. 2011 Jun;85(11):5363–5373. doi: 10.1128/JVI.00115-11

Fig. 4.

Fig. 4.

Analyses of protein profiles in different cell types infected with wild-type and vhs mutant HSV-1. (A) HeLa or Vero cells were mock treated or infected with KOS1.1 or the vhs mutant ΔSma, GFPvhs−, or vhs1. Lysates were prepared at 6 and 12 hpi and analyzed by immunoblotting with antibodies specific for gC, US11, phospho-eIF2α, total eIF2α, β-actin, gB, VP16, ICP34.5, TK, and ICP4. (B) HFF, SK-N-SH, HEp2, HEL, and U2OS cells were mock treated or infected with KOS1.1 or ΔSma. Lysates were prepared 6 and 12 hpi and analyzed by immunoblotting with antibodies specific for gC, US11, phospho-eIF2α, eIF2α, and ICP4. (C) HeLa or Vero cells were mock treated or infected with KOS1.1 or ΔSma. RNA was extracted at 6 and 12 hpi and analyzed by Northern blot with probes specific for gC or US11 mRNA.