Fig. 6.

Polysome analyses. HeLa cells (A, B, C) or Vero cells (D, E, F) were mock treated or infected with KOS1.1 or ΔSma at an MOI of 10. Cells were lysed 12 hpi, incubated with or without EDTA, and analyzed by sedimentation through a 10 to 50% sucrose gradient. Samples were taken from top to bottom, and extracted RNA was analyzed by Northern blotting for the viral mRNAs for gC (A, D) and US11 (B, E) or the cellular γ-actin mRNA (C, F). Bands were quantified by a phosphorimager, and the distribution of the mRNAs across the gradient is shown as a percentage of the total signal of the respective mRNA. The graphs represent one out of three comparable experiments.