Fig. 4.

Analyses of DNA microarrays identify enrichment of genes associated with the NF-κB signaling pathway. (A) Hierarchical clustering of gene expression data from WT and TG prostate tissues. Genes differentially expressed by at least 2-fold were selected for hierarchical clustering. The heat map depicts differential gene expression between the WT and TG groups. Each row represents an individual gene, and each column represents an individual tissue sample. The red and green colors represent relatively higher and lower expression levels, respectively. (B) Summary of real-time PCR data of selected genes identified from DNA array analyses. (C) GSEA with the C3 motif database identified gene sets enriched in the NF-κB pathway and the interferon signaling cascade. FDR, false discovery rate. (D) IHC assays and immunoblotting experiments with anti-p65 antibodies demonstrate increased levels of p65 in the prostate of transgenic mice.