Fig. 4.
Reduced neurotropism of LCMV-GP pseudotypes in vivo. Adult Fischer F344/NCrHsd rats were inoculated intracranially (hippocampus or striatum region) with 7 × 105 TU/ml of VSV-G-complemented or GP-pseudotyped VSV*MQΔG vectors. At 24 h postinfection, brains were removed and suspended in phosphate-buffered saline (PBS)–4% paraformaldehyde. Coronal brain sections (40 μm thick) were prepared. Neurons were counterstained with mouse anti-NeuN (clone A60) and astrocytes with rabbit anti-GFAP (Dako) primary antibody. The respective Alexa Fluor 568-conjugated antibodies were used as secondary antibodies, and nuclear counterstaining was performed with DAPI and TOTO-3 iodide (Invitrogen). (A) Transduction of hippocampal neurons with VSV-G- and GP-pseudotyped vectors. (B) Transduction of striatal neurons by VSV-G- and GP-pseudotyped vectors. (C) Transduction of ependymal cells by VSV*MQΔG-GP. Bars for overview, 400 μm; bars for detail, 50 μm; arrow, injection site; LV, lateral ventricle.