Fig. 4.

The number of RVN inner vesicles is stable when translation is inhibited. Thin sections of SARS-CoV-infected Vero-E6 cells were used to count RVN inner vesicles in DMVs or VPs. For this purpose, images covering a complete slice through the center of the cell were stitched into large mosaics to facilitate the analysis. Per condition, 32 cells from three independent experiments were analyzed. The graph shows the average number of inner vesicles per square micrometer of cytoplasm and illustrates how translation inhibition by either CHX or PUR prevented expansion of the number of inner vesicles as it normally occurs between 4 and 7 h p.i. DMVs were never observed in mock-infected cells. Error bars represent the standard deviations of the averages from three independent experiments.