TABLE 1.
Adult brain and brain stem volumes were measured by MRI for neonatal growth restricted (GR) and control mice with or without neonatal leptin administration. MRI was not performed on leptin-exposed females.
| Male Control | Male GR | Male Control Leptin | Male GR Leptin | Female Control | Female GR | ||
|---|---|---|---|---|---|---|---|
| N | 15 | 12 | 8 | 6 | 7 | 6 | |
| Total Volume | (mm3) | 445 ± 5 | 445 ± 4 | 454 ± 4 | 440 ± 5 | 449 ± 8 | 457 ± 5 |
| Cerebral Cortex | (mm3) | 148 ± 4 | 148 ± 3 | 154 ± 2 | 144 ± 3* | 147 ± 4 | 152 ± 4 |
| (% total) | 33.2 ± 0.4 | 33.2 ± 0.4 | 33.7 ± 0.3 | 32.7 ± 0.3* | 32.7 ± 0.3 | 33.3 ± 0.5 | |
| Brain Stem | (mm3) | 80.8 ± 1.5 | 80.7 ± 1.0 | 83.0 ± 1.1 | 81.0 ± 1.4 | 80.6 ± 1.1 | 84.4 ± 1.1* |
| (% total) | 18.1 ± 0.2 | 18.1 ± 0.1 | 18.2 ± 0.1 | 18.4 ± 0.1 | 17.9 ± 0.1 | 18.4 ± 0.1* | |
| Fiber Tracts | (mm3) | 79.7 ± 1.6 | 78.8 ± 1.3 | 79.8 ± 1.6 | 77.6 ± 1.2 | 83.0 ± 2.3 | 83.7 ± 1.0 |
| (% total) | 17.9 ± 0.2 | 17.6 ± 0.2 | 17.5 ± 0.3 | 17.6 ± 0.2 | 18.5 ± 0.3 | 18.3 ± 0.2 | |
| Cerebellum | (mm3) | 43.2 ± 0.7 | 43.3 ± 1.0 | 44.5 ± 0.5 | 43.5 ± 0.9 | 44.9 ± 0.8 | 43.5 ± 0.8 |
| (% total) | 9.69 ± 0.15 | 9.69 ± 0.20 | 9.77 ± 0.10 | 9.87 ± 0.10 | 10.00 ± 0.09 | 9.51 ± 0.27 | |
| Basal Ganglia | (mm3) | 34.0 ± 0.5 | 34.3 ± 0.4 | 33.0 ± 0.4 | 34.4 ± 0.2* | 33.4 ± 0.4 | 33.2 ± 0.3 |
| (% total) | 7.64 ± 0.21 | 7.69 ± 0.14 | 7.26 ± 0.11 | 7.82±0.10** | 7.45 ± 0.18 | 7.26 ± 0.10 | |
| Hippocampus | (mm3) | 22.7 ± 0.4 | 23.4 ± 0.4 | 22.8 ± 0.6 | 22.8 ± 0.3 | 23.0 ± 0.6 | 21.7 ± 0.3 |
| (% total) | 5.10 ± 0.15 | 5.24 ± 0.13 | 5.01 ± 0.17 | 5.18 ± 0.11 | 5.13 ± 0.19 | 4.74 ± 0.11 | |
| Thalamus | (mm3) | 18.9 ± 0.3 | 18.7 ± 0.2 | 18.6 ± 0.2 | 18.9 ± 0.1 | 18.8 ± 0.2 | 18.9 ± 0.2 |
| (% total) | 4.25 ± 0.12 | 4.19 ± 0.06 | 4.08 ± 0.05 | 4.30 ± 0.07 | 4.18 ± 0.06 | 4.14 ± 0.06 | |
| Hypothalamus | (mm3) | 11.3 ± 0.7 | 11.4 ± 0.6 | 12.7 ± 0.4 | 11.1 ± 0.4* | 11.4 ± 0.8 | 12.5 ± 1.0 |
| (% total) | 2.53 ± 0.14 | 2.54 ± 0.12 | 2.79 ± 0.08 | 2.50 ± 0.06* | 2.53 ± 0.14 | 2.72 ± 0.21 | |
| Ventricles | (mm3) | 3.4 ± 0.2 | 3.2 ± 0.1 | 3.2 ± 0.1 | 3.3 ± 0.1 | 3.2 ± 0.1 | 3.4 ± 0.1 |
| (% total) | 0.76 ± 0.03 | 0.72 ± 0.03 | 0.71 ± 0.01 | 0.75 ± 0.03 | 0.70 ± 0.02 | 0.75 ± 0.03 | |
| Pituitary | (mm3) | 2.1 ± 0.2 | 2.2 ± 0.2 | 2.2 ± 0.1 | 2.1 ± 0.1 | 2.0 ± 0.2 | 2.2 ± 0.2 |
| (% total) | 0.47 ± 0.03 | 0.50 ± 0.04 | 0.48 ± 0.02 | 0.49 ± 0.03 | 0.45 ± 0.04 | 0.49 ± 0.03 | |
| Amygdala | (mm3) | 0.95 ± 0.04 | 1.12±0.07** | 1.01 ± 0.04 | 0.99 ± 0.07 | 0.96 ± 0.05 | 1.08 ± 0.05 |
| (% total) | 0.21 ± 0.01 | 0.25±0.02** | 0.22 ± 0.01 | 0.23 ± 0.01 | 0.21 ± 0.01 | 0.24 ± 0.01 |
*
P<0.05 or
**
P<0.01 versus control.