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In vitro splicing of tetramethylrhodamine-labeled Ad1RNA. Lanes 1–3, unlabeled (TMR−) Ad1 RNA; lanes 4–6, tetramethylrhodamine-labeled (TMR+) Ad1 RNA. The splicing reactions were performed for 0 (lanes 1 and 4), 30 (lanes 2 and 5), and 90 min (lanes 3 and 6). The positions of splicing intermediates and products are indicated at the left of lane 1.
