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The HeLa cells microinjected with various mutant pre-mRNAs (labeled with red fluorochrome) documenting the structural requirements for their targeting to the speckles. After the incubation, the cells were labeled for SC35 protein (in green). The structure of microinjected RNAs is shown. Ad2 RNA (A), AdBS-PPT RNA (B), Ad5 RNA (C), Ad6 RNA (D), Ad8 RNA (E), Ad8++ RNA (F), Ad10 RNA (G), and Ad11 RNA (H). See text for the detailed description.
