Mak2p and Mak3p protect cells against
peroxide attack. Exponentially growing cultures of wild-type (CHP429),
Δprr1 (JM1516), Δprr1
Δatf1 (JM1546), Δmak2 Δmak3 (JM1930), and
Δmak2 Δmak3 Δprr1 (JM2232) cells growing in YES
medium at 30°C were incubated in the same medium containing 50 mM
hydrogen peroxide. Cells were removed at the times indicated, washed,
and plated on YES agar plates to determine the number of viable cells.
The data shown represent the mean values from three independent
experiments.