Table 2.
Conserved amino acid residues and the prediction of transmembrane domains and subcellular localization
| Name | Ar/R selectivity filter | NPAa (LB/LE) | Froger's Position (P1 - P5) | TMHb | Subcellular localization | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H2 | H5 | LE1 | LE2 | P1 | P2 | P3 | P4 | P5 | ||||
| PIP1;1 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP1;2 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 6 | PMc |
| PIP1;3 | F | H | T | R | NPA/NPA | E | S | A | F | W | 6 | PMc |
| PIP1;4 | F | H | T | R | NPA/NPA | E | S | A | F | W | 6 | PMc |
| PIP1;5 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 5 | PMc |
| PIP1;6 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 5 | PMc |
| PIP1;7 | F | H | T | R | NPA/NPA | M | S | A | F | W | 5 | PMc |
| PIP1;8 | F | H | T | R | NPA/NPA | M | S | A | - | - | 5 | PMc |
| PIP1;9 | F | H | T | R | NPA/NPA | M | S | A | - | - | 5 | PMc |
| PIP1;10† | - | H | T | R | NPA/NPA | M | S | A | F | - | - | - |
| PIP1;11 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP1;12† | F | H | T | R | NPA/NPA | E | S | A | F | W | 5 | PMc |
| PIP1;13† | F | H | T | R | NPA/NPA | E | S | A | F | W | 5 | PMc |
| PIP1;14 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP1;15 | F | H | T | R | NPA/NPA | E | S | A | F | W | 6 | PMc |
| PIP2;1 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 6 | PMc |
| PIP2;2 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 6 | PMc |
| PIP2;3 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 6 | PMc |
| PIP2;4 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;5 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;6 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;7 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;8 | V | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;9 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;10 | F | H | T | R | NPA/NPA | Q | S | A | 5 | PMc | ||
| PIP2;11 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 6 | PMc |
| PIP2;12 | F | H | T | R | NPA/NPA | M | S | A | F | W | 6 | PMc |
| PIP2;13 | F | H | T | R | NPA/NPA | Q | S | A | F | W | 6 | PMc |
| TIP1;1 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 6 | Vc |
| TIP1;2 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 6 | Vc |
| TIP1;3 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 6 | Vc |
| TIP1;4 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 6 | |
| TIP1;5 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 7 | Vc |
| TIP1;6† | H | I | A | V | NPA/NPA | T | S | A | Y | W | 6 | - |
| TIP1;7 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 6 | |
| TIP1;8 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 7 | Vc |
| TIP1;9† | H | I | A | V | NPA/NPA | T | S | A | - | - | - | - |
| TIP1;10† | H | I | A | V | NPA/NPA | T | S | A | - | - | - | - |
| TIP1;11† | H | I | A | V | NPA/NPA | T | S | A | - | - | - | - |
| TIP1;12† | H | I | A | V | NPA/NPA | T | S | A | - | - | - | - |
| TIP1;13† | H | I | A | V | NPA/NPA | T | S | A | - | - | - | - |
| TIP1;14 | H | I | A | V | NPA/NPA | T | S | A | Y | W | 7 | Vc |
| TIP2;1 | H | I | G | R | NPA/NPA | T | S | A | Y | W | 7 | PM/Vc |
| TIP2;2 | H | I | G | R | NPA/NPA | T | S | A | Y | W | 6 | Vc |
| TIP2;3† | H | I | G | R | NPA/NPA | T | S | A | Y | W | 7 | - |
| TIP2;4 | - | I | G | R | NPA/NPA | T | S | A | Y | W | - | Vc |
| TIP2;5† | - | I | G | R | -/- | T | S | A | Y | W | - | |
| TIP2;6 | H | I | G | R | NPA/NPA | T | S | A | Y | W | 6 | Vc |
| TIP2;7 | H | I | G | R | NPA/NPA | T | S | A | Y | W | 6 | Vc |
| TIP4;1 | H | I | A | R | NPA/NPA | T | S | A | Y | W | 6 | PMc |
| TIP4;2 | H | I | A | R | NPA/NPA | T | S | A | Y | W | 6 | PMc |
| NIP1;1 | W | V | A | R | NPA/NPA | F | S | A | Y | L | 6 | PMc |
| NIP1;2† | W | V | A | - | NPA/NPA | F | - | - | - | - | - | - |
| NIP1;3† | W | V | A | R | NPA/NPA | F | S | - | - | - | - | - |
| NIP2;1 | G | S | G | R | NPA/NPA | L | S | A | Y | V | 5 | PMc |
| NIP5;1† | A | I | G | R | NPS/NPV | F | T | A | Y | L | - | - |
| NIP5;2† | A | I | G | R | NPS/NPV | F | T | A | Y | L | - | - |
| NIP6;1† | T | I | A | R | NPA/NPV | F | T | A | Y | F | 6 | - |
| NIP6;2† | T | I | A | R | NPA/NPV | F | T | A | Y | F | 6 | - |
| NIP6;3† | T | - | - | - | NPT/- | - | - | - | - | - | - | - |
| NIP6;4† | T | - | - | - | NPT/- | - | - | - | - | - | - | - |
| NIP6;5† | T | - | - | - | NPA/- | F | - | - | - | - | - | - |
| NIP6;6† | T | I | A | - | NPA/NPV | F | - | - | - | - | - | - |
| SIP1;1 | F | I | P | F | DPA/NPA | I | A | A | Y | W | 5 | PM/Gc,ERe, Sd |
| SIP1;2 | I | T | P | N | NPT/NPA | M | A | A | Y | W | 5 | ERe, Sd |
| SIP1;3 | V | T | P | N | NPT/NPA | L | A | A | Y | W | 5 | ERe |
| SIP1;4 | V | T | A | S | NPA/NPA | I | A | A | Y | W | 3 | Vc, ERe |
| SIP1;5† | V | T | P | N | NPT/NPA | L | A | A | - | - | - | - |
| SIP1;6† | V | T | P | N | NPT/NPA | L | A | A | - | - | - | - |
| SIP1;7† | V | T | A | R | NPA/NPA | I | A | A | - | - | - | - |
| XIP1;1 | I | T | V | R | NPV/NPA | V | C | A | F | W | 7 | PM/Vc |
†: Partial sequence.
a: Bold italic letters denote unusual amino acids in NPA motifs.
b: Number of transmembrane helices (TMH) predicted by TMHMM analysis tool [64].
c: PSORT (PM, plasma membrane; C, cytoplasm; V, vacuole; G, golgi) [65].
d:TargetP (S, secretion)[66].
e: Prodotar (E, endoplasmic reticulum) http://urgi.versailles.inra.fr/predotar/predotar.html.
-: Not determined due to limited sequence information.