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18:3-Glu is rapidly metabolized on wounds of N. attenuata leaves. Three lines of puncture wounds were generated on leaves with a fabric pattern wheel and (a) 0.17 nmoles of synthetic 18:3-Glu, or (b) 10 μL M. sexta OS (containing approximately 0.17 nmoles of 18:3-Glu) were applied onto the wounds. Leaves were extracted at different times and 18:3-Glu levels quantified by LC-MS/MS. Initial 18:3-Glu amounts (T0 ↑) were set at 100%. Bars represent standard errors (± SE, n = 4).
