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. 2010 Aug 9;10:164. doi: 10.1186/1471-2229-10-164

Figure 2.

Figure 2

Radio-HPLC chromatograms of wounded N. attenuata leaves treated with 14C-18:3-Glu. (a) Leaves of rosette stage N. attenuata plants were wounded with a pattern wheel and 0.1 μCi of [1-14C]18:3-Glu were immediately applied onto leaf wounds. The damaged tissue was harvested immediately (T0) or 2 min after the treatment. After extraction, the radiolabeled metabolites were separated by RP-radio-HPLC. The numbers (1 to 3) indicate the different fractions collected for further analysis. CPM: counts per minute. (b) M. sexta OS was spiked with 0.1 μCi 14C 18:3-Glu, applied onto wounded leaf tissue, extracted after different time points and separated by TLC (W+OS). Plates were exposed for 24 h to 14C-sensitive screens. Time 0: 14C 18:3-Glu was applied onto wounded leaves and immediately extracted. W+: 14C-18:3-Glu was applied onto wounded leaves and extracted after 2 min. 18:3-Glum1 and 18:3-Glum2 indicate different modified forms of 18:3-Glu.